Multiplex qPCR is a popular area of interest for many assay developers. Thanks to distinct reporter dyes, multiple reactions can be combined into a single well, with each target detected independently ...

Screening greater than 10 pathogens or SNPs in a sample is challenging due to limitations in fluorescence colors in a multiplex quantitative PCR. Optimizing a multiplex qPCR is daunting, and it limits ...

A recent study posted to the medRxiv* preprint server reported the development and validation of a two-reaction multiplex reverse transcription-quantitative polymerase chain reaction (RT-qPCR) ...