本研究通过头对头比较Vircell、Certest和Altona三种HDV RNA qRT-PCR检测试剂盒，揭示了其在定性诊断上高度一致（κ=0.988-1.000），但存在显著的定量偏倚（Vircell和Certest分别高估0.33和0.24 log10IU/mL）。同时，研究证实热休克（thermal shock）前处理虽能提升低病毒载量样本的 ...
Ideally, gene expression studies by quantitative RT-PCR (qRT-PCR) should be performed using RNA from homogeneous cell populations. Frequently, this means performing qRT-PCR using picogram amounts of ...
本研究针对冷冻浆果中人类诺如病毒（HuNoV）检测依赖qRT-PCR技术无法区分病毒活性的问题，以鼠诺如病毒（MNV）为替代模型，通过ISO 15216:2017方法系统分析病毒载量与感染性的关联。结果表明qRT-PCR检测灵敏度（1.0 log PFU/25 g）较细胞培养法（3.1 log PFU/25 g）高2个 ...
COVID-19 is commonly diagnosed by quantitative fluorescence real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) (1, 2). The test is the sequential action of two enzymes–an ...
Breast cancer in a patient with multiple endocrine neoplasia type 1 (MEN 1): A case report and review of the literature. This is an ASCO Meeting Abstract from the 2012 ASCO Annual Meeting I. This ...
Lumpectomy plus tamoxifen with or without irradiation in women age 70 or older with early breast cancer. Background: Gene expression profiling has reclassified breast cancer into intrinsic subtypes ...
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