A new preprint describes a single-step molecular diagnostic test for the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection caused by the Omicron variant that is behind ...

The COVID-19 pandemic has resulted in demand for population scale pathogen screening. The most sensitive and reliable method for detecting SARS-CoV-2 remains reverse transcription PCR (RT-PCR).

For decades, optimizing annealing temperatures in PCR has pushed the limits of PCR forward to more difficult tasks. At the same time, mastermixes and kits became more powerful and less prone for ...