弓形虫病活动性感染的Naica Crystal ddPCR系统评估显示与qPCR符合率97%，但样本基质效应导致阈值波动和可分性评分差异，影响结果解读。定量分析显示qPCR Ct值与ddPCR拷贝数呈负相关（R2=0.80）。样本类型（胎盘、脑脊液等）和保存条件影响ddPCR性能，成本较高限制 ...

Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...