Studier's T7 expression system uses the T7 promoter to "turn on" a gene of interest and the T7 RNA polymerase to transcribe that gene into messenger RNA (mRNA) so that E. coli ribosomes can use the ...

Specific contacts between the Escherichia coli RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) and the phosphates and purine bases of the A3 promoter of phage T7 cluster ...