Show how to use restriction mapping to determine the orientatio... | Filo

SOLVED: Construct a restriction map of a linear fragment of DNA, using the following data Your map should indicate the relative positions of the restriction sites along with distances from the ends of the molecule to the restriction sites and between restriction sites:DNA uncut DNA DNA cut with EcoRI DNA cut with Hindlll DNA cut with BamHI DNA cut with EcoRI Hindlll DNA cut with EcoRI BamHI DNA cut with Hindlll BamHISizes of Fragments (bp) 900 700, 200 600, 300 500,350, 50 600,200, 100 500, 200,

SOLVED: The figure below shows a restriction map of a piece of DNA containing your favorite gene. The arrow indicates the position and orientation of the gene in the DNA. In part (B) of the figure are enlargements showing the portions of the DNA whose sequences have been used to make oligonucleotide probes A, B, C, and D. Which of the oligonucleotides can be used to detect the gene in each of the following?(A)(B)A. A Southern blot of genomic DNA cut with HindIII.B. A Northern blot.

a. Given the following restriction map of a cloned 10 kb piece of DNA, what size fragments would you see after digesting this linear DNA fragment with each of the enzymes or combinations of enzymes listed? (1) EcoRI, (2) BamHI, (3) EcoRI + HindIII, (4) BamHI + Pstl, and (5) EcoRI + BamHI. b. What fragments in the last three double digests would hybridize on a Southern blot with a probe made from the 4 kb Bam HI fragment? | Numerade

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