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AP Biology: Restriction Enzyme Digests on Circular Plasmids
This video describes how to analyze restriction enzyme digests on circular plasmid DNA. Emphasis is placed on predicting the number and size of fragments for a given digest. This is problem set #1 in a series. If you want additional practice, check out problem set #2: https://youtu.be/Wred9wH9KEA
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SOLVED: Texts: A restriction map lists the locations of DNA sequences that are cut by a particular restriction enzyme for a piece of DNA, such as a chromosome or a plasmid. Restriction maps are important when generating a construct for experimental use. Digesting the DNA sequence with the restriction enzymes will result in fragmented DNA of predictable sizes, based on the restriction map, that allow a researcher to analyze if his or her construct was generated correctly when visualized using gel
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